Extraction of DNA from onions - 1450 Words

EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes, DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate, namely: Dische Test, Murexide Test, Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic acid) was discovered in the late 1800s, but its role as the material of heredity was not elucidated for fifty years after that. It occupies a central and critical role in the cell as the genetic information in which all the information required to†¦show more content†¦The heat softens the membrane as a whole. This entire step, homogenation, is used to free the DNA from its protective barriers. Then crude papain was added, because Deproteinization which involves adding a protease enzyme Papain will denature the proteins clinging to the DNA making the molecule flexible and easy to spool. Then the solution was then heated again to 60 degrees for 10 minutes with occasional stirring. Then it was placed in an ice bath, this is because by cooling the solution, it will help prevent denaturation, which might destroy the DNA if it was exposed to prolonged heating. Swirling was done in order not to shock the DNA from the sudden change in temperature. After putting it in an ice bath, the solution was then placed in a blender and was blended for 45 seconds, after. This was done because the procedure also frees the DNA from another protective barrier, the thick cell wall that all plant cells have. The blending should cause many of the cell walls, containing cellulose, to break, and therefore leave the DNA in the cytoplasm, and no protective thick cellulose wall blocking the cytoplasm from its external environment. We did not need to do this to the bacteria because it does not have a cell wall. The blended solution was then filtered through 4 layers of cheesecloth leaving the foam untouched. This is because itShow MoreRelatedThe Forensic Analysis Of Dna972 Words   |  4 Pagesof the Forensic Analysis of DNA Introduction All living beings possess DNA that is unique to them.2 For this reason DNA is often used in criminal investigations to help ascertain a perpetrators identity1. In this lab we intend to mimic the methodology involved in such a process. DNA consists of thymine, guanine, cytosine and adenine in a double helix structure, in a sequence that is unique to each person.2 A lysis solution is to be used to safely isolate onion DNA from tissue, consisting of sodiumRead MoreExtracting Onion Dna1346 Words   |  6 PagesExtraction of DNA from an Onion Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants. DNA was discovered in the 1950’s, there still remains a lot to be known about it, especially how it is used to determine the physical traits that we all have, and how it regulates the workings of the body. deoxyribonucleic acid is a chemical, we can do reactions with it just like we can work with any other chemical. Experiment: Note: Read MoreSimilarities And Differences Between Haploid And Haploid Cells Essay1578 Words   |  7 Pageschromosomes, essentially chromosomes are compacted chromatins as they both carry genetic information in the appearance of genes. Contrast: Chromosomes are a condensed chromatin fibers that are higher up in the order of DNA. Chromatins are composed of nucleosomes and are lower in the order of DNA. Chromosomes are compact, thick and coiled while chromatin is comprised of thin and long fibers that are uncoiled in their structure unlike chromosomes. Chromosomes are paired together while chromatin are unpairedRead MoreBio 101 Essay24965 Words   |  100 Pages               Lab  5:  Ã‚  The  Chemistry  of  Life   Lab  6:  Ã‚  Diffusion   Lab  7:  Ã‚  Osmosis   Lab  8:  Ã‚  Respiration   Lab  9:  Ã‚  Enzymes        Ã‚  The  Cell:      Lab  10:  Ã‚  Cell  Structure  Ã‚  Function                  Lab  11:  Ã‚  Mitosis   Lab  12:  Ã‚  Meiosis   Lab  13:  Ã‚  DNA  Ã‚  RNA   Lab  14:  Ã‚  Mendelian  Genetics   Lab  15:  Ã‚  Population  Genetics         3   Common  Labware  found  in  ESL  Kits      4      Lab  Safety   Always  follow  the  instructions  in  your  laboratory  manual  and  these  general  rules:   eScience  LabsRead MoreSexually Transmitted Diseases35655 Words   |  143 PagesPhysical Environment Weather and Climate 100-113 Soils 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on populationRead MoreJournal Of Essential Oil Bearing Plant : Article Report5034 Words   |  21 PagesEssential Oil Bearing Plants Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/teop20 Effect of Time Distillation on Chemical Constituents and Anti-Diabetic Activity of the Essential Oil from Dark Green Parts of Egyptian Allium ampeloprasum L. Yasser A. Selimab Mohamed I. Sakerancd a Faculty of Specific Education, Zagazig University, Zagazig,44519, Egypt b Department of Basic Clinical Medical Science, Faculty of Dentistry, P. QaseemRead MoreQuality Improvement328284 Words   |  1314 PagesIndustrial Engineering and Statistics and the Arizona State University Foundation Professor of Engineering. He received his B.S., M.S., and Ph.D. degrees from Virginia Polytechnic Institute, all in engineering. From 1969 to 1984 he was a faculty member of the School of Industrial Systems Engineering at the Georgia Institute of Technology; from 1984 to 1988 he was at the University of Washington, where he held the John M. Fluke Distinguished Chair of Manufacturing Engineering, was Professor of Mechanical

Persuasive Essay On Police Brutality - 1471 Words

Over the years, the United States has faced many cases of police brutality. Officers are faced with many threatening situations everyday forcing them to make split-second decisions expecting the worse, but hoping for the best. Therefore, police brutality severely violates human rights in the United States. Police officers have one of the hardest jobs America has to offer. They have to maintain public order, prevent, and identify crime. Throughout history, the police community has been exposed by violence in some way or another. Police officers have been suspects in dangerous shootings, severe beatings, fatal chokings, and unnecessary killings. The history of police brutality is repeated, going through stages of violence, corruption, and†¦show more content†¦Three years later, 5’5ft 145lb Johnny Gammage was pulled over while driving through a mostly white Pittsburgh neighborhood, and was choked and beaten to death apparently after attacking five white police officers. In 1997, a New York City police officer shoved a stick six inches into the rectum of a guy named Abner Louima cracking his intestines (Troutt 6). To make things even worse the officer stuck the stick into the victims mouth afterwards. On April 30, 2014 Milwaukee police officer Christopher Manney fatally shoots Dontre Hamilton, an unarmed 31-year-old African American with a history of mental illness, in a downtown park. Manney alleged that Hamilton, who appeared to be homeless, attempted to grab his baton during a pat down. Manney says he shot Hamilton 14 times in self-defense. Manney was fired in October but was not charged in the shooting. On Nov. 22, 2014, Tamir Rice, 12, is fatally shot and killed in a Cleveland park after police responded to a 911 call reporting a person with a gun. The caller warned that the gun may have been fake, but the officers say they didn’t know that. Officer Timothy Loehmann shot Rice within seconds of arriving on the scene. Rice’s gun tur ned out to have been a toy. A group of political and religious leaders have called for criminal charges to be brought against the officers involved, and a grand jury plans to hear evidence in the caseShow MoreRelatedPersuasive Essay On Police Brutality1400 Words   |  6 PagesThe fear of being killed by the ones who are supposed to protect you just keeps growing in the United States. Yes this essay is about the brushed off topic of police brutality and how it’s got to be stopped before it gets even way more out of hand. It’s just scary to think that the people who are supposed to protect you have a never ending list of just names and ages which they were responsible for killing. The ages of the deaths go to as young as 14 to all the way to 70 the lives of kids and elderlyRead MorePersuasive Essay On Police Brutality892 Words   |  4 PagesPolice officers once were called peace keepers however now they seem to be nothing more than law enforcing officers. Most still do their jobs but they are more concerned about quotas and making arrests on Innocent people rather than keeping peace. Which as a following result has brought up a lot of apprehensiveness between the police and their citizens. With increasing violence in cities and states, police officers methods have slowly become more aggressive, bringing a rise in unnecessary policeRead MorePersuasive Essay On Police Brutality1079 Words   |  5 Pagessimplest procedures such as a traffic stop. Most do not even want to encounter a policer officer. The reason why? Police brutality. The article Police Brutality by gale defines police brutality as: The use of unnecessary, excessive force by police in their encounters with civilians. The force used is beyond what would be considered necessary in the situation at hand. (Police Brutality). The same individuals who are supposed to protect and serve the citizens of the United States have instilledRead MorePersuasive Essay On Police Brutality1663 Words   |  7 PagesPolice officers primary responsibility is to protect and serve citizens and communities, not to abuse the power that they were given to hurt innocent people. For personal enjoyment or a personal vendetta. Some states have the â€Å"Stand-Your-Ground† laws, which allows innocent citizens the right to use deadly force to defend and protect themselves. But what if they were protecting themselves from police brutality. Police brutality can be has been going on for many years. But recently has become a biggerRead MorePersuasive Essay On Police Brutality880 Words   |  4 PagesOnce â€Å"A young nigga got it bad ‘cause Im brown, and not the other color, so police think, they have the authority to kill a minority. F*ck that Sh*t cause I ain’t the one.† (NWA, 1988). Today’s police brutality is ridiculous. Police brutality is the use of excessive and/or unnecessary force by police when dealing with civilians. Every other week a loved black citizen is taken away from us. Over the past decade, police have acted out in ways that have made people wonder if our officers of the lawRead MorePersuasive Essay On Police Brutality1893 Words   |  8 PagesAmerican JusticeDue to the growing abundance of evidence clearly demonstrating that brutality and racism are prominent issues within the United States police system, coupled with the fact that the majority of officers who commit such crimes are never indicted, the Federal government must adopt certain reforms to remedy this situation. There are countless proposals and ideas aimed at solving this issue of police brutality, but there is one formulation of plans that would seem to be the most effectiveRead MorePersuasive Essay On Police Brutality1467 Words   |  6 PagesHow can we trust the ones that’s killing us? Law enforcement and police officers play a major role in our community. They help those in need keep us safe. Nationwide they also kill an average of 3 people every day. I thought whenever you become an officer, you have sworn to protect us. Police brutality is a major issue in our society today. Police work is dangerous. In the past, police officers were considered peacekeepers. Police have been occupied in shootings, severe beatings, and unnecessarilyRead MoreEnglish 1A Essay 3 1 3 1624 Words   |  7 PagesSomer 13 November 2014 The Horrors of Police Brutality Imagine being in the Bart train, going to a party with some of your friends, but while in route, you are shot by the Bart police for a crime you did not commit. This is the story of a young man named Oscar Grant. On New Years Eve of 2009, he was fatally shot. . Police brutality is the use of excessive force, physically or verbally, by a police officer. In one year, how many incidents of police brutality or misconduct do you think have occurredRead MoreThe Use Of Brutality And Persuasion1626 Words   |  7 PagesThe use of brutality and persuasion in interviews by Police. In this case analysis it will discuss the purpose of interrogatory deception when conducting interviews, ways in which it is used, some of the current debates over the practice, and examples of theories to explain corruption and brutality. The number one priority that should be thought about prior to any form of interrogation Interviewing is the suspect’s rights and privacy are to be respected. However in some cases police have failedRead MoreThe Reality Of Alleged Police Misconduct2036 Words   |  9 PagesApril, 2017 The Reality of Alleged Police Misconduct The idea of police brutality being a major problem in the United States has many people wondering if the police are there on the streets to protect the citizens, or to abuse them. Police brutality in the United States is not the problem. â€Å"Brutality Isn’t the Problem, It’s People Who â€Å"Disrespect Our Authority† (Grigg). Many individuals in the United States have the idea that it is all right to override a police officer’s authority that’s given to

Competency Of MNC HR Director Management †Myassignmenthelp.Com

Question: Discuss About The Competency Of MNC HR Director Management? Answer: Introduction Todays economy is globally driven which has added immense weight to roles of HR professionals. Huge responsibilities are dispensed to an HR director of a MNC and this requires HR directors to have some particular competencies which are in relevance with the changing and emerging HR practices. This write up has its basis on the given case study which seeks competency of MNC HR director. Little confusion surfaces when it comes to acceptance of significance regarding theoretical and empirical basis of global HRM with practical application. Scholars like Armstrong, Taylor (2014) argued with an air of optimism that even though the HRM in global context walks in infancy level but still these have vibrancy with diverse nature triggering growth in development of theory and practices of HR in the earlier decades. The case study of the MNC has assortment of businesses that transcend its country of origin. The business of export and global IT consulting moves with the changing curve of foreign direct investment. Considering the FDI flow, the business remains largely confined to emerging countries like India, China with focus of business on central European nations. The overall business runs with rationale but has strong effort to stay restricted to somewhat which is defined as significant in relation to Global HRM (Alfes et al, 2013). Traditional HR theories like result orie ntation, strategic collaboration, development of employee competence remain spatial with relatively advanced direction inspired from emerging practices. In this context, the business highly soaks up HR models like Harvard model and the Warwick model. Into the vision of the business the dimensions in this model fits better as stakeholders interests, HR policy selection, HR results and situational factors. The Warwick model helps the business to think on content of business strategy, micro and macro environmental aspects and HR practices accordingly (Guest, 2011). The business activities and the HR practices therein demands some competencies in the HR director and these are strategic thinking capacity, capacity in challenge mitigation, policy making knowledge, great level of HR knowledge including practical and theoretical insight, implementation of most relevant HR practices on time. Business of the MNC with the developed trade outlook struggles to cope with cultural differences. The proposition of the fast development of emerging economies with adequate focus on global HRM is important in several ways (Jiang et al, 2012). Its impact on setting of global trade far exceeds the possibilities as regions for outsourced activities of less value aspects of MNC. The MNC with their roots in Europe feel challenges in having synergies among business performances and manpowers contribution. Cultural differences in India, China and central and Western Europe differ a lot due to which a coordinated HR practices dither in taking place. The competency needs of HR director remains maximum here in implementing a well coordinated common HR practice across the segments of cultural differences and to make better working places with maximum devotion of employees with shared goals of the company. Conclusion Strategic dimension of business and organisational capacity building is pivotal to long term growth of the company. HR director must comply with the criteria and the competencies that are in want, for driving business towards success. With more potential prosperity, lays there the latent challenges that can be controlled with requisite level of expertise in the directorial position. References Armstrong, M., Taylor, S. (2014). Armstrong's handbook of human resource management practice. Kogan Page Publishers. Alfes, K., Shantz, A. D., Truss, C., Soane, E. C. (2013). The link between perceived human resource management practices, engagement and employee behaviour: a moderated mediation model. The international journal of human resource management, 24(2), 330-351. Guest, D. E. (2011). Human resource management and performance: still searching for some answers. Human resource management journal, 21(1), 3-13. Jiang, K., Lepak, D. P., Hu, J., Baer, J. C. (2012). How does human resource management influence organizational outcomes? A meta-analytic investigation of mediating mechanisms. Academy of management Journal, 55(6), 1264-1294.